Complete a subculture

A subculture moves the same line on a round: tissue cut from this line's jars onto fresh media. The line keeps its ID; its round number goes up.

1. Open the line and choose Complete subculture.
2. Check the date and pick the new media batch (batches the protocol suggests are starred), or keep the current one.
3. Under New jars, say how many jars each source jar went into. Every jar → with Apply to healthy jars sets them all at once.
4. Note explants, shoots, the stage after transfer, and anything you observed. Turn on contamination if you found any.
5. Save subculture, then Print labels for the new jars, numbered by their path.

At the bench, on the phone

Start subculture session on a line (or Subculture from this jar on a scanned jar) lets you scan each jar as you cut it, enter how many it went into, then Finish and quick-print the new labels.